Journal: Vaccines
Article Title: Construction of Candida albicans Adhesin-Exposed Synthetic Cells for Preventing Systemic Fungal Infection
doi: 10.3390/vaccines11101521
Figure Lengend Snippet: Schematic illustration ( a ) and characterization of the synthetic E. coli strains Em and EmEAP1. ( a ) A scheme indicating the construction of the strains and the induction of protein exposure in the synthetic cells. ( b ) Confocal images of the Em and EmEAP1 cells after induction of IPTG for 12 h. The cells were stained by DAPI (blue fluorescence, indicating bacterial DNA) and the FITC (green fluorescence)-conjugated anti-Eap1 body for observation. The mCherry field indicates the synthetic mCh/mChEap1 expressed by the E. coli cells. ( c ) Quantification of the FITC fluorescence intensity of the stained cells. The asterisk indicates a significant difference between the two groups ( p < 0.05).
Article Snippet: The E. coli strain BL21 (the chassis) was obtained from GenScript (GenScript Biotech Corp., Nanjing, China).
Techniques: Staining, Fluorescence